Effect of inhalation exposure to dimeforce organophosphate pesticide on the histology of the brain of wistar rats

Abstract

This study examined the impact of inhalation of dime force pesticide on histology of the brain of Wistar rats. A total of twenty (20) rats weighing (80-142g) were divided into four groups of five rats each. The control group received feed and water only, whereas groups (2,3,4) were exposed once daily for 30 minutes (low-dose), 45 minutes (medium-dose), and 1 hour (high-dose) respectively for 14 days. At exactly 24 hours following the last treatment, animals were anaesthetized using chloroform and the brain was extracted from each group, fixed and taken for histological analysis. The results on the morphology of the brain revealed distinct alterations in cerebellar tissue, Purkinje cell loss, mild to moderate vacuolation, and other structural disruptions across all the exposed groups as compared to the control. These findings suggest that Dimeforce exposure at certain concentrations or durations may induce mild to moderate pathological changes in the brain, however, the degree of brain damage may depend on exposure levels, route and duration, which underscores the importance of adoption of safety measures during applications in agriculture and for occupationally exposed populations.