Dynamism in Pro-inflammatory Cytokines and Hormonal Changes of Mice Induced Endometritis Treated with Aqeous Extract of Asphilia Africana

Abstract

Reproduction is one of the key pillars of the production in many herds and individual animal in female animal, reproduction can be affected by uterine disease including metritis and endometritis. To this effect, this study investigated the effect of Asphilia africana on the treatment of endometritis. Thirty mice were randomly assigned into 5 groups: control group, infectious group, treatment A, treatment B, treatment C. After acclimatization, the 4 treatment were induced endometritis with the use of lipopolysaccharide. (LPS 0.114 b3e.coli) through the vulva. Asphilia africana were administered to the mice from the low dosage (0.01ml) medium dosage (0.03ml) and high dosage (0.05ml) for the treatment of endometritis. At the end of the induction, blood sample was collected randomly from the mice to analyse the inflammatory cytokines and hormonal change using specific Enzyme-linked immunosorbent assay (ELISA) kit; data collected were analyze using analysis of the variance (ANOVA). Base on the finding of the study, it was concluded that the high dosage of Asphilia africana had great effect on induced endometritis of mice, hormonal changes and pro inflammatory cytokines and it could be recommended that the varying dosage of Asphilia africana could reduce the rate of reproductive hormone abnormalities and postpartum inflammation. Therefore could be used cautiously in therapeutic procedures.